Courses:

  • Vibrational Spectroscopy in Biophysics
    Code: NBCM017
    Range in summer semester: 0/6 Z

  • Practical Course in Physics III for General Physics majors
    Code: NOFY028
    Range in summer semester: 0/4 Z
    Annotation: Praktikum z optiky. Podrobnější informace naleznete zde
    Note: odborná asistence, odpovědný vyučující: Mgr. Hana Císařová

  • Importance and Functions of Metal Ions in Biological Systems
    Code: NBCM023
    Range in winter semester: 2/0 Zk
    Annotation: Inorganic elements in living systems, their occurrence and function. Essentiality and toxicity of metals. Complex ions of transient metals. Interactions of the metals with porphyrins, nucleic acids and proteins. Metabolism of the most important metals (Fe, Cu, Zn, Ni) and the most important enzymes containing trace elements. Chemotherapeutics containing some non-essential metals.

  • Optical Microscopy and Selected Imaging Techniques in Biophysics
    Code: NBCM114
    Range in winter semester: 2/0 Zk

  • Curiosities in Optics
    Code: NUFY064
    Range in winter semester: 0/2 Z

  • Experimental methods of biophysics and chemical physics I
    Code: NBCM177
    Range in winter semester: 4/0 Zk
    Annotation: Sources, detectors and spectrum analyzers in optical spectroscopy Interaction of optical radiation with an isolated molecule. Dipole approximation Selection rules for electronic, vibrational and rotational optical transitions. Methods and applications of electron absorption spectroscopy. Excitation and sounding method. Use of polarized radiation and its analysis in optical spectroscopy. Linear and circular dichroism. Methods and applications of vibrational absorption spectroscopy. Methods of elastic, dynamic and Brillouin scattering and their use. Raman scattering, methods of measurement and use. Principles and basic concepts of luminescence (types of luminescence, Jablonsky diagram, kinetics, quantum yield, lifetimes, Franck-Condon principle). Influence of intermolecular interactions on luminescence parameters (influence of environment, resonant energy transfer, emission quenching). Emission anisotropy Measurement of stationary and time-resolved luminescence. Phosphorescence, single-molecular spectroscopy. Influence of interaction with the environment on the width of the spectral line. Nuclear magnetic resonance: principle, experimental setup, excitation and signal detection, basic pulse sequences. High resolution NMR of organic substances in liquids: interpretation of spectra. Electron paramagnetic resonance: principle, experimental setup, application. Scattering and diffraction of X-rays, electrons and neutrons. Principles of basic diffraction methods. Symmetry and structure of crystals and their determination from the diffraction pattern.

  • Experimental methods of biophysics and chemical physics II
    Code: NBCM178
    Range in summer semester: 2/0 Zk
    Annotation: Fluorescent markers and probes; Fluorescent proteins; protein fluorescence Atomic force microscopy and plasmon resonance Mössbauer spectroscopy Calorimetry Mass spectrometry Photoacoustic spectroscopy Separation methods (centrifugation, chromatography, electrophoresis) Electron microscopy Electrochemistry (cyclic voltammetry, electrochemical impedance spectra.) Basics of MRI imaging and microimaging

  • Advanced methods of optical spectroscopy
    Code: NBCM179
    Range in summer semester: 3/0 Zk
    Annotation: Advanced imaging methods (FLIM, polarized and spectral FLIM, spectral imaging). Time-resolved frequency domain fluorescence spectroscopy, FCS. Fluorescence, confocal, multiphoton and supercompression microscopy. Nonlinear methods of Raman scattering (HRS, SRS, CARS), Raman optical activity (ROA). Advanced techniques of Raman spectroscopy (SERS, CRM, DCDR). Generation and characterization of femtosecond pulses. Fundamentals of 2DES spectroscopy. Nonlinear optical phenomena and their use in optical spectroscopy (parametric generation, generation of higher harmonics, Kerr and Pockels effect. High spectral resolution methods. Low temperature spectroscopy. Relaxation processes, transverse and longitudinal relaxation time, homogeneous width and lifetime, spectra in a solid matrix.