Optimizing the method of oligonucleotide concentration determination by UV absorption spectroscopy

Author: Mgr. Barbora Profantová (roz. Řezáčová), Ph.D.
Supervisor: Prof. RNDr. Josef Štěpánek, CSc.
Type: Bachelor

Abstract: This work deals with problems of precise determination of the oligonucleotide (short section of nucleic acid) concentration by means of UV absorption measurement. Nowadays oligonucleotides and their analogs represent promising chemotherapeutics for the strategy of harmful gene silencing. As accurate as possible knowledge of their concentrations in the solutions under study is required to determine correctly their physico-chemical properties. Our work investigates systematically individual influences upon results of UV absorption measurements and compares the procedures recommended in literature according to their reliability and accuracy.

Keywords: None