Study of biomolecules using SERS spectroscopy on solid metal nanosubstrates

Author: Mgr. Hana Šípová, Ph.D.
Supervisor: Prof. RNDr. Josef Štěpánek, CSc.
Consultants:
Type: Master

Abstract: Main goal of this Bachelor Thesis was to test possibilities of application of surface-enhanced Raman scattering (SERS) and confocal Raman microscopy to biomolecular study. Obtained spectra have been compared with previously measured ones using macro-Raman spectrometer. Copper phtalocyanine as testing molecule and gold colloidal nanoparticles immobilized to glass plates as SERSactive surfaces has been chosen. Our results show that confocal Raman microspectrometer provides spectra with higher signal-to-noise ratio. Other advantage is easier manipulation and substantially shorter accumulation times. In addition, confocal system allows us to remove strong Raman signal of glass. Finally, it is necessary to give attention to possible sample decomposition in the case of Raman microscope.

Keywords: None