Metal particles attached to silanized glass plates and their application as SERS-active substrates in study of biomolecules

Author: Mgr. Natália Hajduková
Supervisor: Prof. RNDr. Marek Procházka, Dr.
Consultants:
Type: Master

Abstract: Main goal of this work was to develop a methodology of preparing gold nanoparticles attached to silanized glass plates and their application in study of selected biomolecules using surface-enhanced Raman scattering (SERS). Stable, homogenous and reproducible substrates suitable for SERS spectroscopy have been prepared using silane with functional amino group, gold nanoparticles prepared by chemical reduction of HAuCl4 by sodium citrate and their þheat treatmentÿ after immobilization. SERS spectra of positively and negatively charged porphyrin in its native unmetalated form in broad concentration range (from 10-7 to 10-4 M) as well as the spectra of some water insoluble molecules without interference of the solvent have been obtained from these substrates.

Keywords: SERS, gold nanoparticles, porphyrins, metalation