Non-resonant Raman Spectroscopic Study of Guanine Quadruplex Structures

Annotation: Guanine quadruplexes are non-canonical four-stranded structures formed under certain conditions from initially single-stranded regions of telomeric DNA rich in guanine residues. Depending on the number, orientation and details of the base sequence of the interacting oligonucleotides, there exists wide range of structurally and conformationally diverse quadruplexes. Guanine quadruplexes are widely studied notably because of their potential anticancer function. Diploma thesis will deal with formation, structure and stability of guanine quadruplexes by means of non-resonant Raman spectroscopy. The aim of the thesis will be determination of conformational markers typical of various types of guanine quadruplexes. All necessary equipments, methodologies and materials for diploma thesis are arranged. Own initiative, self-activity, reliability and vigorous approach in solving the problems are expected from the candidate.