Finished grants:

  • Studium polymorfismu kvadruplexů nukleových kyselin

    Grant agency: GAUK
    Identification number of grant: 388615
    Head of the project: Mgr. Kateřina Mudroňová
    Vice-head of the project: Mgr. Jakub Klener, Doc. RNDr. Peter Mojzeš, CSc., Václav Římal ()

    Grant annotation

  • UV resonance Raman scattering of nucleic acids

    Grant agency: GAUK
    Identification number of grant: 691412
    Head of the project: Mgr. Jakub Klener
    Vice-head of the project: Prof. RNDr. Josef Štěpánek, CSc.

    Grant annotation
    The project is focused on determination of secondary and tertiary structures of nucleic acids (NA) and their interactions. Considering the requirement of measurements in natural aqueous environment, Raman spectroscopy (RS), which is capable of acquiring basic information about the structure and structural changes of NA and their complexes, is very convenient for that purpose. The disadvantage of standard RS with visible excitation is the necessity of high NA concentration and the problems with contamination of samples by low-molecular impurities. These difficulties can be overcome by using resonance Raman scattering (RRS) with excitation in the long-wavelength NA absorption band. Because of substantially higher demands on the apparatus, its adjustment and employment of measuring schemes that protect the sample from photodegradation, this technique is only rarely used in NA studies. The main task of the project will be, besides building up the UV RRS spectrometer and development of suitable procedures for handling with UV photosensitive samples, to find out RRS markers sensitive to NA conformation and its interaction. These findings will be applied in a subsequent study of particular NA structural motives, important for the transfer and expression of genetic information.