New approaches for study of protein structure by means of vibrational spectroscopy
Grant agency: GAAS
Identification number of grant: KJB101120805
Head of the project: RNDr. Vladimír Kopecký Jr., PhD.
Vice-head of the project:
RNDr. Kateřina Hofbauerová, PhD.
Grant annotation
Determination of the protein structure represents one of the key tasks
of present molecular biology. There is an urgent need for
tree-dimensional structures of proteins for understanding their role in
organisms and efficient drug design. The main goal of the project is
development of the new methodology for estimation of the secondary
structures content of proteins by means of vibrational spectroscopy
(Raman spectroscopy, Raman optical activity, drop deposition-coating
Raman spectroscopy, Fourier-transform infrared spectroscopy and
Fourier-transform infrared spectroscopy with attenuated total
reflection). The large protein reference spectra set for each method
will be measured, present methods will be implemented and new methods
(based on multivariate statistic) with higher information content, e.g.
determination of the number of structure segments, of the protein fold
etc., will be developed. The methods will be freely available and can be
applied in future protein research.
