Interactions of disulfide groups in peptide/ protein molecules - complementary use of electronic and vibrational optical activity
Grant agency: GACR
Identification number of grant: P205/10/1276
Head of the project: Prof. RNDr. Vladimír Baumruk, DrSc.
Vice-head of the project:
Ing. Petr Maloň, CSc.
Grant annotation
We will study impact of mutual interactions of disulphide groups in
peptides and proteins. We would like to verify already established
relationship between disulphide geometry and chiroptical properties
(electronic circular dichroism - ECD and Raman optical activity - ROA)
under the circumstances when more than one disulphide group is present
in a molecule. Moreover, possibility to detect and conformationally
analyze disulphide groups in a presence of amide groups will be
evaluated. We also plan to investigate magnitude of mutual interactions
in electronic spectra (number of dichroic bands and their splitting) and
possibilities and limitations of observation of individual disulphide
groups on the basis of C-S and S-S- stretching vibrational bands in
Raman and particularly ROA spectra.
