Grants:

  • Signaling pathways affecting human TRPC5 receptor function: Prediction of their association with rheumatoid arthritis pain

    Grant agency: GACR
    Identification number of grant: 22-13750S
    Head of the project: RNDr. Viktorie Vlachová, DrSc. (Fyziologický ústav AV ČR, v. v. i.)
    Vice-head of the project: RNDr. Ivan Barvík Jr., PhD., Tomáš Soukup ()

    Grant annotation

    Rheumatoid arthritis (RA) is a painful systemic autoimmune disease severely afflicting around 1% of the worldwide population. The genetic profile of RA patients emerges as a key determinant of therapeutic efficacy and a number of gene polymorphisms associated with RA related signaling pathways have been identified. Recently, genetic or pharmacological blockage of transient receptor potential canonical 5 (TRPC5) has been shown to amplify joint inflammation and hyperalgesia in human and murine models of RA; however, the cellular mechanisms await elucidation. Using a combination of molecular biology, microscopy, electrophysiology, and molecular modeling, this project aims to (i) screen for fundamental cellular mechanisms regulating human TRPC5 channel, (ii) genotype single nucleotide polymorphisms along the intracellular pathways involved in RA with the aim to clarify the putative role of TRPC5 as the target channel, and to (iii) characterize the molecular mechanisms through which TRPC5 may contribute to the pathogenesis of rheumatoid arthritis pain and inflammation.

Finished grants:

  • The role of the carboxyl-terminal domain in polymodal activation of the ankyrin receptor TRPA1

    Grant agency: GAUK
    Identification number of grant: 426311
    Head of the project: Mgr. Vlastimil Zíma
    Vice-head of the project: RNDr. Ivan Barvík Jr., PhD., Mgr. Lucie Surá (Fyziologický ústav AV ČR, v. v. i.), RNDr. Viktorie Vlachová, DrSc. (Fyziologický ústav AV ČR, v. v. i.)

    Grant annotation
    The ankyrin transient receptor potential 1 channel (TRPA1) is a transmembrane protein which plays a key role in the transduction of noxious chemical and thermal stimuli by peripheral nociceptors. From the cytoplasmic side, TRPA1 is critically modulated by calcium ions which permeate through the channel and modulate the channel by an as-yet-unknown mechanism (s). The aim of this project is to - characterize the functional role of the carboxyl-terminal tail of TRPA1 in chemical and voltage-dependent activation - understand the mechanisms underlying the voltage-dependent modulation by focusing on the conserved negatively charged loop between the C-terminal putative helices 4 and 5 - explore to what extent this loop participates in the modulation of TRPA1 by calcium ions - explore the extent of functional and structural homology between this region in TRPA1 and the calcium-binding region in the BK subtype of potassium channels - create model of tertiary structure of TRPA1 C-terminus which will be used for studying interaction with calcium ions at atomic resolution Knowledge of these underlying mechanisms is a prerequisite for obtaining a better understanding of the structure and function of TRPA1 and for the future development of more specific pain therapies.

  • Antibakteriální látky nové generace

    Grant agency: MSMT
    Identification number of grant: 2B06065
    Head of the project: Mgr. Libor Krásný, PhD. ()
    Vice-head of the project: RNDr. Ivan Barvík Jr., PhD., Ing. Dominik Rejman, PhD. (), MUDr. Otakar Nyč (), Mgr. Tomáš Látal ()

    Grant annotation
    None

  • Inhibitory bakteriální RNA polymerázy založené na analozích nukleotidů

    Grant agency: MSMT
    Identification number of grant: NR9138
    Head of the project: Mgr. Libor Krásný, PhD. ()
    Vice-head of the project: RNDr. Ivan Barvík Jr., PhD., Ing. Dominik Rejman, PhD. (), MUDr. Otakar Nyč (), Mgr. Tomáš Látal ()

    Grant annotation
    None

  • Molecular dynamics simulations of chemically modified nucleic acids - potential chemotherapeutics

    Grant agency: GACR
    Identification number of grant: 202/02/D114
    Head of the project: RNDr. Ivan Barvík Jr., PhD.

    Grant annotation
    None